Data/hora: 18/05/2024 06:17:02

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	08/02/2021	
Data da última atualização: 	09/02/2021	
Autoria: 	LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; NAVARRO, J. S.	
Afiliação: 	MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚSA. SÁNCHEZ NAVARRO, Universidad Politécnica de Valencia.	
Título: 	Unravelling the involvement of cilevirus p32 protein in the viral transport.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Scientifc Reports, n.11, v.2943, 2021.	
Idioma: 	Inglês	
Conteúdo: 	Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread.	
Thesagro: 	Doença de Planta; Fruta Cítrica.	
Thesaurus Nal: 	Citrus; Plant diseases and disorders.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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